Cmv Promoter Size


ADDGENE: PROMOTERS
Updated 54 years ago
FREE From addgene.org
Promoters Definition A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase to DNA. RNA … ...

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ADDGENE: VECTOR DATABASE - CMV PROMOTER
Updated 54 years ago
FREE From addgene.org
CMV promoter Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … ...
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ADDGENE: PCMV-GFP
Updated 54 years ago
FREE From addgene.org
CMV promoter-EGFP Alt name cytomegalovirus immediate early promoter Insert Size (bp) 1400 Tag / Fusion Protein EGFP (C terminal on insert) Cloning Information Cloning method Restriction Enzyme 5′ cloning site … ...

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COMPARISON OF THE EF-1 ALPHA AND THE CMV PROMOTER FOR …
Updated 54 years ago
FREE From pubmed.ncbi.nlm.nih.gov
In contrast in clones carrying the CMV promoter, only up to 60% of the cells were GFP-positive with expression levels varying widely between cells. Superinfection with wild … ...
Author Christian Teschendorf, Kenneth H. Warrington, Dietmar W. Siemann, Nicholas Muzyczka
Publish Year 2002

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CMV AND T7 PROMOTERS | RESEARCHGATE
Updated 54 years ago
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CMV is typically a "stronger" promoter and recruits more transcriptional machinery at a greater rate than T7, it is also eukaryotes. There is a good summary of many different promoters... ...

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CMV PROMOTER (ZV924N) | DOULIX - GET STARTED
Updated 54 years ago
FREE From doulix.com
The CMV promoter does not seem to work in T24 and HCV29 human bladder urethelium carcinoma cell lines but SV40 (part no. GA-pro-00020.1) exhibits high activity in these … ...

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OPTIMIZING PROMOTERS FOR RECOMBINANT ADENO-ASSOCIATED …
Updated 54 years ago
FREE From pubmed.ncbi.nlm.nih.gov
In a comparative study of commonly used 800-bp cytomegalovirus (CMV) and chicken β-actin (CBA) promoters, we report significant differences in the patterns of cell-specific … ...

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ENGINEERING OF THE CMV PROMOTER FOR CONTROLLED …
Updated 54 years ago
FREE From onlinelibrary.wiley.com
Apr 28, 2022 This engineered CMV promoter displayed a ∼ 14% increase in expression (p = 0.005; Figure S2) while being 25% smaller in size compared to the wild-type CMV. To illustrate the enhanced capability of … ...

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CYTOMEGALOVIRUS - WIKIPEDIA
Updated 54 years ago
FREE From en.wikipedia.org
For instance, the double‑stranded DNA (dsDNA) genome of wild-type HCMV strains has a size of around 235 kb and encodes at least 208 proteins. It is thus longer than all other human herpesviruses and one of … ...

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HUMAN CMV IMMEDIATE-EARLY ENHANCER: A USEFUL TOOL TO ENHANCE …
Updated 54 years ago
FREE From pubmed.ncbi.nlm.nih.gov
Conclusions: The results of this study indicate that lentiviral vectors with the human CMV enhancer conferring efficient cell-type-specific gene expression may be useful tools for … ...

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IMPACT OF DIFFERENT PROMOTERS ON EPISOMAL VECTORS HARBOURING ...
Updated 54 years ago
FREE From nature.com
May 26, 2016 Setting the length of the UBC promoter to a value of 100 and normalizing the other promoter lengths to this, promoter length was ruled out as a factor … ...

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RETRACTED ARTICLE: IMPACT OF DIFFERENT PROMOTERS, PROMOTER
Updated 54 years ago
FREE From nature.com
Sep 5, 2017 In the present study, six commonly used promoters, including cytomegalovirus major immediate-early (CMV), the CMV enhancer fused to the chicken beta-actin … ...

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CAG PROMOTER - WIKIPEDIA
Updated 54 years ago
FREE From en.wikipedia.org
The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors. [1] [2] CAG promoter was … ...

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DEVELOPMENT OF A NEW PROMOTER TO AVOID THE SILENCING OF GENES IN …
Updated 54 years ago
FREE From jbioleng.biomedcentral.com
Jun 28, 2019 While the CMV promoter was initially more powerful than RegCG, the latter promoter was more resistant to silencing than the CMV promoter in stable cell lines, … ...

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PROMOTER CHOICE: WHO SHOULD DRIVE THE CAR IN T CELLS?
Updated 54 years ago
FREE From journals.plos.org
Jul 24, 2020 In addition EF-1 and CMV predominantly enriched for TFs specific or highly expressed in T cells [27–29, 32, 33] such as GATA3, NFATc3, NF-kB, AP1 and c-Jun, … ...

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CMV ENHANCER MAY NOT BE SUITABLE FOR TISSUE-SPECIFIC ... - NATURE
Updated 54 years ago
FREE From nature.com
May 27, 2019 After the CMV enhancer was inserted before the AFP promoter, the AFP promoter activity was increased significantly in these cell lines (from 32-fold to 1096 … ...

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CONSTRUCTION OF AN EXPRESSION VECTOR MEDIATED BY THE DUAL …
Updated 54 years ago
FREE From link.springer.com
Jun 20, 2020 The simian vacuolating virus 40 (SV40) promoter, cytomegalovirus (CMV) promoter and elongation factor 1α (EF1α) promoter are highly effective promoters for … ...

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WHY IS THERE A DIFFERENCE BETWEEN THE EF1A PROMOTER …
Updated 54 years ago
FREE From researchgate.net
Jun 23, 2017 The core EF1a promoter is only about 200-300bp in size. The 1172bp version actually contains an intronic sequence, which is occasionally spliced out. This extra sequence gives a higher level of... ...

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IMPROVED TET-RESPONSIVE PROMOTERS WITH MINIMIZED BACKGROUND …
Updated 54 years ago
FREE From bmcbiotechnol.biomedcentral.com
Nov 24, 2010 The CMV-minimal promoters differ in size, but both contain the authentic TATA-box and initiator sequence (Inr). (C) The newly designed Ptet promoters are … ...

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